ActimTMPROM, AmniSure®, and ROM +plus®: Rupture of membrane kits tested on amniotic fluid from women at C-section: a comparative study

Objectives: To confirm three point of care commercial tests for rupture of membranes consistently and accurately detect amniotic fluids from pregnant women, and they are practical and easy to use. Methods: Samples of fresh amniotic fluid were collected in a syringe taken at time of Csection and run on kits (ActimTMPROM, AmniSure®, and ROM +plus®) from three commercially available point of care rupture of membrane tests. Samples were sent to an independent laboratory for ELISA analysis of these amniotic fluid proteins (insulin-like growth factor binding protein (IGFBP-1) and alpha-Fetal protein (AFP). Test results of known amniotic fluid on ActimTMPROM, AmniSure®, and ROM +plus®, along with ease of use of comments. False negatives were used to determine accuracy of tests. Results: Correct results were obtained in 96.8%, 95.8%, and 98.9% respectively for ActimTMPROM, AmniSure®, and ROM +plus®. Users reported ROM +plus® was easiest to use. Conclusions: ROM +plus®. provided the least amount of false negative testing on known samples of amniotic fluid. ROM +plus® was considered most simple and easiest to use and detects two different amniotic fluid proteins while the other tests detect one protein.